Journal: bioRxiv

Article Title: Role of Ribeye PXDLS/T-binding cleft in normal synaptic ribbon function

doi: 10.1101/2023.12.12.571266

Figure Lengend Snippet: INS-1 cells were transfected with rat Ribeye-GFP and then immuno-stained for Ribeye and several presynaptic proteins. (A-D) show example images from co-staining for Ribeye and Piccolo (A), RIM2 (B), CaV1.3 (C) and Bassoon (D). For each set of images, magenta denotes the presynaptic protein of interest, green indicates GFP and the right panel merges both colors. Note the striking degree of colocalization between spots of Piccolo and GFP in A; scale bar: 5µm applies to all images. (E) Colocalization was quantified by determining the ICQ value. The ICQ varies from −0.5 to 0.5, with the higher numbers indicating better correlation between the two channels. For each protein pair, an ICQ value was determined for Piccolo n=17, RIM2 n=14, CaV1.3 n=4 and Bassoon n=6 transfected cells images and averaged. *P<0.05.

Article Snippet: The primary antibodies used for cell staining and zebrafish were CtBP [CtBP (B-3, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA)], RIM2 (anti-Rim2 PDZ domain, Synaptic Systems, Goettingen, Germany), CaV1.3(L-type Ca++CP, H160, Santa Cruz Biotechnology, USA), Bassoon (ab76065, Abcam, Cambridge, MA, USA), and c-myc (9E10) (sc-40, Santa Cruz Biotechnology, Inc., Santa Cruz, CA, USA) and Piccolo (Anti-Antibody [6H9-B6] and StressMarq Biosciences INC; Anti-Piccolo antibody (ab20664) Abcam, Cambridge, MA, USA), anti-green fluorescent protein mouse IgG1(Invitrogen A11121); anti-green fluorescent protein mouse IgG2a(Invitrogen 1037264)GFP monoclonal Antibody(clontech632375) were diluted 1:200.

Techniques: Transfection, Staining

Journal: bioRxiv

Article Title: RAB3 phosphorylation by pathogenic LRRK2 impairs trafficking of synaptic vesicle precursors

doi: 10.1101/2023.07.25.550521

Figure Lengend Snippet: Plasmids and reagents

Article Snippet: Plasmid: CMV HA-RIM2 , This paper , Addgene #206151.

Techniques: Recombinant, Plasmid Preparation, Knock-Out, Transfection, Modification, Bicinchoninic Acid Protein Assay, Software, Imaging

Journal: Science Advances

Article Title: Resolving the molecular architecture of the photoreceptor active zone with 3D-MINFLUX

doi: 10.1126/sciadv.abl7560

Figure Lengend Snippet: Antibody list. aa, amino acids; Ms, mouse; ihcs, inner hair cells.

Article Snippet: Rabbit anti-RIM2 , aa 909–1076 rat RIM2-4C (UniProt ID: Q9JIS1-3 ) , 140 303, Synaptic Systems; Ms rods ( ) and Ms ihcs ( ) .

Techniques: